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Latest news

July 16th, 2025: Congratulations to Dr. Cancade-Veyre!

Bravo Lison for an excellent Viva and obtaining the PhD. Intense and exciting discussion on Brucella, effectors and nucleomodulins with the jury members Jean Celli (University of Vermont, USA), Melanie Hamon (Institut Pasteur, Paris), Stephan Kohler (IRIM, Montpellier, France), and Patricia Doublet (University of Lyon, France).

 

July 8th, 2025: Congratulations to Dr. Debruyne!

Charline defended her PhD work and obtained the well-deserved PhD degree!

Excellent discussion on all aspects of intracellular Acinetobacter baumannii with the evaluation committee Javier Pizarro-Cerda (Institut Pasteur, France), Kimberley Kline (University of Geneva, Switzerland), Stephan Göttig (University of Franckfurt, Germany) and Karen Moreau (University of Lyon, France).

 

July 2025: Day out to celebrate several important lab milestones!

 

A mix of hiking, relaxation, sightseeing and lots of laughs. An opportunity to visit some of the beautiful spots in Wisconsin.

 

June 2025: Our latest preprint is now available. Extensive work by Charline Debruyne to characterize the intracellular niche of clinical Acinetobacter baumannii strains.

Human endothelial cell infected with Acinetobacter baumannii ABC141 strain. Bacteria can be seen in white, as the nucleus of the cell, thanks to the DNA stain Dapi. The mitochondria are in red. One can see a cluster of bacteria indicative of extensive intracellular multiplication.In collaboration with the Kröger lab and Karsten Hokamp at Trinity College, Charline mapped the bacterial and host gene expression profiles of strain ABC141 in endothelial cells. She also defined this transient intracellular compartment suitable for multiplication as segregated from degradative lysosomes and showed bacteria egress to efficiently infect neighboring cells.  A surprising ability of ABC141 to grow in low oxygen was also noted as she studied an atypical hypoxia gene signature detected in infected cells.

Congratulations Charline!

 

June 2025: Congratulations to Lison Cancade-Veyre for her first author publication!

A small but very nice story about undertaking the study of the molecular interactions between the Brucella abortus Nyx effectors. Lison showed NyxA and NyxB form a complex in cells and highlighted a role for their dimerization during infection. And she got invited to prepare the cover!

Check it out at this link 

 

Thanks to our collaborators Laurent Terradot (MMSB, Lyon, France) and Francine Gerard (MMSB, Lyon, France) and to Arthur Louche that initiated the work a while back.

 

28th May 2025: EMBO Conference Cell biology of host-pathogen interactions, Blois, France

Meeting old friends and making new ones after a week of outstanding host-pathogen interactions talks.

Going back with so many ideas of cell biology experiments to be done for the Brucella Nyx effector project.

Thank you Hubert Hilbi and Lena Pernas for the invite!

 

 

April 2025: Finally, the Notice of Award for the lab’s first NIH grant arrived!

Exciting work ahead to study the role of the NADase activity of the Brucella TIR domain-containing proteins in immunomodulation and disease pathology in collaboration with the team of Dr. Judith Smith, here at UW-Madison.

 

 

April 2025: Wonderful visit to Washington University in St. Louis – Thank you Mario Feldman for hosting me!

Spent a wonderful few days visiting the Molecular Microbiology Department at Washington University.

Great discussions with faculty and students. We also received excellent feedback and suggestions for our current Brucella project.

Had a blast visiting St. Louis with Mario.

 

February 2025: T4SS meeting in Nancy, France

A week of excellent talks and posters,

and lots of effector discussions with the Celli, deBolle, Dehio, Terradot and Bonazzi labs present and all working on Brucella T4SS.

Final gala dinner was fun, in a beautiful setting with a very entertaining magician.

Thank you to the organizers Badreddine Douzi and Nicolas Soler for the invite. I was honored to give a keynote in such an outstanding meeting.

 

December 2024: Suzana visited the Department of Microbiology and Immunology at East Carolina University

Exciting few days listening to excellent science, meeting great students and postdocs and lots and lots of talking and thinking about one of our favorite bugs: Brucella.

Thank you Marty Roop for hosting.

 

 

 

 

Online talk at the Portuguese Society of Microbiology (Portugal-Brasil joint conference)

Suzana presented the lab’s work on intracellular Acinetobacter baumannii in this exciting online meeting with lots of student and postdoc talks.

 

The Salcedo lab enjoying the last summer warmth at the UW-Madison terrasse

 

September 2024: Lab in full force at the Midwest Microbial Pathogenesis Conference at Indiana University, Bloomington.

Charline and Lison presented posters on Acinetobacter and Brucella, respectively and Suzana gave a talk on a Pseudomonas TIR domain protein!

 

 

August 2024: Landon Hodge presents his work at the Veterinary Scholars Symposium in Minneapolis

Well done, Landon! In such a short time, Landon got lots of exciting results with the Acinetobacter baumannii bovine isolates and also succeeded in screening some bacterial genes for their role in adhesion, invasion, and intracellular multiplication of human isolate ABC141. Congratulations on mastering the genetics of our difficult multi-drug resistant strains!

Thank you Boehringer Ingelheim, for funding Landon with a Veterinary Summer Scholars Grant.

 

 

June 2024: Welcome to Landon Hodge, Summer Scholar SVM student

Landon has joined our lab for the summer to help identify the bacterial genes involved in intracellular Acinetobacter baumannii and study recent veterinary clinical isolates from Wisconsin. We thank SVM for this excellent program and Boehringer Ingelheim for funding Landon with a Veterinary Summer Scholars Grant.

 

May 2024: Talk at UIC Chicago and visit of the Department of Microbiology and Immunology

First US science trip since arriving here. Thanks to Lauren Palmer for hosting and to all those I met in the department.

 

January 2024: New publication on the importance of taxonomy: Ochrobactrum Is Not Brucella!

Moreno E et al. (2023) If You’re Not Confused, You’re Not Paying Attention: Ochrobactrum Is Not BrucellaJournal of Clinical Microbiology 61(8): e00438-23. doi: 10.1128/jcm.00438-23

 

IBA meeting in Oslo, Norway

Suzana participates at the IBA National Graduate School in Infection Biology and Antimicrobials meeting in Oslo, Norway (October 2023). Excellent oral and poster presentations from the graduate students.

 

Charline participates in the Cold Spring Harbor meeting

Charline presenting her poster

Charline Debruyne participates in the Microbial Pathogenesis and Host Response Meeting at the Cold Spring Harbor. She presented a poster on the intracellular niche of Acinetobacter baumannii clinical strains.

 

The Salcedo Lab has moved!

Suzana by the Vet School Bucky

The Salcedo Lab has moved from France to the University of Wisconsin-Madison, School of Veterinary Medicine!
We are just getting started preparing our lab space to welcome everyone. Charline and Lison will stay a bit longer in the lab of our long-time friend and collaborator Matteo Bonazzi at IRIM in Montpellier.

Time for a new website, lab name and logo…

Logo and lab name: intrapath

 

 

 

 

News archive

Congratulations to our collaborators Stephan Göttig et al. for beautiful work showing the impact of Acinetobacter baumannii on mitochondria. We are excited to have contributed to this nice story!

Leukert L, Tietgen M, Krause FF, Schultze TG, Fuhrmann DC, Debruyne C, Salcedo SP, Visekruna A, Wittig L, Göttig S. (2023) Infection of Endothelial Cells with Acinetobacter baumannii Reveals Remodelling of Mitochondrial Protein Complexes. Microbiology Spectrum  Jun 15;11(3):e0517422. doi: 10.1128/spectrum.05174-22.

Goodbye party

July 2023

Goodbye party to close the lab in Lyon! Eleven wonderful years of collaborations, friendships, lots of laughs, and a few tears.

 

Graduate student dinner

June 2023

Lab dinner with previous graduate studentsWonderful dinner with all the previous graduate students from the lab: Stephanie Gagne, Jean-Baptiste Luizet, Arthur Louche, Morgane Roussin Lison Cancade-Veyre, Charline Debruyne and Amandine Blanco. Just missing Paul Imbert who is now in London and that I met a couple of weeks after.

 

 

Just published, the Nyx effectors targeting nuclear functions

January 2023

Just published! Kudos to Arthur and Amandine for this achievement.

Composite image showing the detection of translocated Nyx in infected cells, the structural domain relevant for its function, the delocalization of nucleolar proteins and of SENP3.Extensive collaborative work identifying two new Brucella effectors targeting the host nuclear functions. We have named them Nyx, in honor of the Greek goddess of the night, daughter of Chaos, to illustrate the scientific path to their discovery!
We highlight that NyxA and NyxB display a novel nucleomodulatory function that promotes perturbation of the subcellular localization of nucleolar proteins during bacterial infection. Structural studies identified the key interacting domains mediating Nyx targeting of the host de-SUMOylase SENP3, needed for efficient intracellular multiplication of Brucella abortus. Click here for article.